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Technology to Analyze Glycan Patterns Directly from Glycoproteins
The world's first selective ionization technology for glycans that does not require pretreatment
(This is a technology for which Hokkaido University is the sole applicant and sole inventor.)We have discovered the world's first mass spectrometry technique for selective ionization of glycans in complex macromolecules and mixtures such as glycoproteins and body fluids by the MALDI method. We have also demonstrated that this technique can be used for the direct analysis of glycans in complex mixtures such as egg white and body fluids.
Research
Glycan patterns on glycoproteins are important biomarkers because they are factors that determine the disposition of protein in the body. Until now, glycan pattern analysis has required complicated operations such as cutting, chemical modification, and purification of glycans. Mass spectrometry is an ultra-sensitive and high-resolution analytical technique that can directly ionize trace amounts of biomolecules. However, there has not been a method to selectively ionize glycoconjugates such as glycoproteins and glycans in complex macromolecules and mixtures such as body fluids, which requires the complicated pretreatment described above. We have achieved the world's first simultaneous selective cleavage and selective ionization of glycoconjugate glycans, and succeeded in the direct analysis of glycan patterns on glycoproteins. We have also demonstrated that this technique can be used to directly analyze glycan patterns in complex mixtures such as egg white.
Hiroshi Hinou Professor ProfessorPh.D.
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- Analysis of Thermo-acoustic Vibration Generated by Combustion Equipment Osamu Fujita (Professor)
- Gel that is Stronger Than Steel Takayuki Kurokawa (Professor)
- Discovery and Application of a Novel Enzyme Capping the N-Terminus of Peptides Tohru Dairi (Professor)
- Remote Sensing of Ground Deformation in the Arctic Masato Furuya (Professor)
- Liquid Atomization Technology Using Ultrasound and Microbubbles Kazumichi Kobayashi (Associate Professor)