Hokkaido University Research Profiles

Japanese

Pathogens: 1

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  • Simple Pathogen Measuring Devices

    Pathogens can be measured by simply adding samples to the kit.

    We have developed technology that allows measurement of the concentration of E. coli, coliforms, and enterococci within as early as 1 hour and as late as 12 hours after simply adding 0.1 mL of a sample (sewage, wastewater, food extract, drinking water) to the measurement kit and installing it in the device.

    Research

    Currently, the measurement of pathogenic bacteria requires a much time and labor, including the preparation of agar media, multiple dilutions of a large amount of sample, and 24-hour incubation. We have developed a kit that can measure pathogenic bacteria (fecal contamination indicator bacteria) such as E. coli, coliforms, and enterococci simply by injecting 0.1 mL of liquid sample or food extract. Compared to the current general bacteria measurement technology, this is an extremely simple technology that requires only mixing of the sample with the solution. The bacterial concentration is measured using a fluorescent dye. The fluorescent dye can be used even if the liquid is turbid, so the concentrations of various bacteria, even in turbid samples such as wastewater or food extracts, can be measured directly without any pretreatment.