Content of research

Since immunoassay is an essential technology in clinical laboratories and for environmental analysis, technological advances (simplification, high sensitivity, high throughput, etc.) has come essential as the analytes are becoming more diverse and smaller. We aim to construct an ultra-sensitive immunoassay system in which the bioluminescent enzyme Luc is encapsulated in lipid bilayer membrane vesicles, called liposomes, and use it as a label in immunoassays. By encapsulating a large number of Luc in liposomes, it becomes possible to label antibodies in a stable state. However, although the method of encapsulating enzymes inside liposomes is already known, no studies have been done on Luc. In our study, we have encapsulated heat-stabilized recombinant Luc into liposomes and evaluated the amount and stability of the encapsulation. We have also constructed an immunoassay system for C-reactive protein using Luc-encapsulated liposomes as a label.

Potential for social implementation

• ・Clinical testing of ultra-trace markers
• ・Analysis of environmental pollutants
• ・Immunoassay using microdevices

Appealing points to industry and local governments

We aim to improve the sensitivity and functionality of bioluminescence analysis methods in molecular assembly fields. In this research, we have to overcome some more issues to achieve ultra-high sensitivity. We would like to collaborate with companies that are interested in using this method. We also request technical support from companies.